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Your Position: Start > Antibody > Bevacizumab > BEB-Y12

Anti-Bevacizumab Antibody (AY12) (recommended for neutralizing assay)

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  • Source

    Anti-Bevacizumab Antibody (AY12) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    AY12

  • Species

    Mouse

  • Isotype

    Mouse IgG1/kappa

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Human

  • Immunogen

    Bevacizumab.

  • Specificity

    Recognizes Bevacizumab specifically.

  • Application
    ApplicationRecommended Usage
    ELISA0.1-100 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Bevacizumab SDS-PAGE

Anti-Bevacizumab Antibody (AY12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Bevacizumab ELISA

Anti-Bevacizumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development. Immobilized bevacizumab at 5 µg/mL, add increasing concentrations of Anti-Bevacizumab Antibody (AY12) (Cat. No. BEB-Y12, 10% human serum) and then add biotinylated bevacizumab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 62 ng/mL (QC tested).

 Bevacizumab ELISA

Demonstration of the specificity of Anti-Bevacizumab Antibody (AY12) (Cat. No. BEB-Y12) to the bevacizumab.

 Bevacizumab ELISA

Measured by its neutrlizing ability in a functional ELISA. Immobilized bevacizumab at 2 μg/mL (100 μL/well) can bind pre-mixed Anti-Bevacizumab Antibody (AY12) (Cat. No. BEB-Y12) and Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) with a inhibition rate of 96%.

Bioactivity-SPR
 Bevacizumab SPR

Anti-Bevacizumab Antibody (AY12) (mouse IgG1, Cat. No. BEB-Y12) captured on CM5 chip via anti-mouse antibodies surface, can bind human bevacizumab with an affinity constant of 0.08 nM.

  • Background
    A recombinant humanized monoclonal IgG1 antibody that binds to and inhibits the biologic activity of human vascular endothelial growth factor (VEGF). Bevacizumab contains human framework regions and the complementarity-determining regions of a murine antibody that binds to VEGF. Bevacizumab is produced in a Chinese Hamster Ovary mammalian cell expression system in a nutrient medium containing the antibiotic gentamicin and has a molecular weight of approximately 149 kilodaltons.
  • Clinical and Translational Updates

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