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Your Position: Start > Antibody > Adalimumab > ADB-Y19

Anti-Adalimumab Antibody (AY19)

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  • Source

    Anti-Adalimumab Antibody (AY19) is a Mouse monoclonal antibody produced from a hybridoma created by fusing SP2/0 myeloma and Mouse B-lymphocytes.

  • Clone

    AY19

  • Species

    Mouse

  • Isotype

    Mouse IgG1/kappa

  • Antibody Type

    Hybridoma Monoclonal

  • Reactivity

    Human

  • Immunogen

    Adalimumab.

  • Specificity

    Recognizes Adalimumab specifically.

  • Application
    ApplicationRecommended Usage
    ELISA5-600 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Adalimumab SDS-PAGE

Anti-Adalimumab Antibody (AY19) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Adalimumab ELISA

Anti-Adalimumab Antibodies bridging ELISA for Anti-Drug Antibody (ADA) assay development.Immobilized adalimumab at 1 µg/mL, add increasing concentrations of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19, 10% human serum) and then add biotinylated adalimumab at 5 µg/mL. Detection was performed using HRP-conjugated streptavidin with a sensitivity of 0.6 ng/mL (QC tested).

 Adalimumab ELISA

Demonstration of the specificity of Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) to the adalimumab.

 Adalimumab ELISA

Measured by its neutralizing ability in a functional ELISA. Immobilized adalimumab at 0.5 μg/mL (100 μL/well) can bind pre-mixed Anti-Adalimumab Antibody (AY19) (Cat. No. ADB-Y19) and Biotinylated Human TNF-alpha, His,Avitag (Cat. No. TNA-H82E3) with an inhibition rate of 100%.

Bioactivity-SPR
 Adalimumab SPR

Anti-Adalimumab Antibody (AY19) (mouse IgG1, Cat. No. ADB-Y19) captured on CM5 chip via anti-mouse antibodies surface, can bind human adalimumab with an affinity constant of 1.36 pM.

  • Background
    Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant DNA technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
  • Clinical and Translational Updates

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