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Human Integrin alpha 9 beta 1 (ITGA9&ITGB1) Heterodimer Protein, His Tag&Tag Free

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  • Synonym
    Integrin alpha 9 beta 1,ITGA9 & ITGB1
  • Source
    Human ITGA9&ITGB1 Heterodimer Protein, His Tag&Tag Free(IT1-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 30 - Trp 981 (ITGA9) & Gln 21 - Asp 728 (ITGB1) (Accession # Q13797-1 (ITGA9) & P05556-1 (ITGB1)).
    Predicted N-terminus: Tyr 30 (ITGA9) & Gln 21 (ITGB1)
  • Molecular Characterization

    Human ITGA9&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA9 and ITGB1, has a calculated MW of 111.1 kDa (ITGA9) and 82.7 kDa (ITGB1). Subunit ITGA9 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (NR) protein migrates as 210-240 kDa respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha 9 beta 1 SDS-PAGE

Human ITGA9&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit. It is the non-covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits. The integrin alpha 9 beta 1 mediates cell adhesion to tenascin-C and VCAM-1 by binding to sequences distinct from the common integrin-recognition sequence, arginine-glycine-aspartic acid (RGD). A thrombin-cleaved NH(2)-terminal fragment of osteopontin containing the RGD sequence has recently been shown to also be a ligand for integrin alpha 9 beta 1.
  • Clinical and Translational Updates

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