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Your Position: Start > Protein > CD28H > CDH-H5251

Human CD28H / IGPR-1 Protein, Fc Tag (MALS verified)

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  • Synonym
    TMIGD2,IGPR1
  • Source
    Human CD28H, Fc Tag(CDH-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Gly 150 (Accession # AAH15655.1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    CD28H Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.6 kDa. The protein migrates as 45-60 kDa under reducing (R) condition, and 90-130 kDa,150kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
CD28H SDS-PAGE

Human CD28H, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
CD28H MALS images

The purity of Human CD28H, Fc Tag (Cat. No. CDH-H5251) is more than 90% and the molecular weight of this protein is around 86-106 kDa verified by SEC-MALS.

Bioactivity-ELISA
 CD28H ELISA

Immobilized Human B7-H7, His Tag (Cat. No. B77-H52H6) at 5 μg/mL (100 μL/well) can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.6-10 ng/mL (QC tested).

Bioactivity-BLI
 CD28H BLI

Loaded Human B7-H7, His Tag on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 16.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 CD28H BLI

Loaded Cynomolgus B7-H7, His Tag (Cat. No. B77-C52H3) on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 13.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD28 homolog (CD28H), also called transmembrane and immunoglobulin domain containing 2 (TMIGD2) and IGPR-1,is encoded by the TMIGD2 gene. CD28H is constitutively expressed on naive T and NK cells. After interaction of CD28 homolog with B7 homologue,the peripheral effector and memory T cells can be activated and proliferated by Akt-dependent signalling cascade. Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.
  • Clinical and Translational Updates

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