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Your Position: Start > Protein > IFN-alpha/beta R2 > IF2-H5224

Human IFN-alpha/beta R2 Protein, His Tag (MALS verified)

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  • Synonym
    IFNAR2,IFNARB,IFNABR,IFN-R-2,IFN-alpha,beta receptor 2
  • Source
    Human IFNAR2, His Tag(IF2-H5224) is expressed from human 293 cells (HEK293). It contains AA Ile 27 - Lys 243 (Accession # P48551-2).
    Predicted N-terminus: Ile 27
  • Molecular Characterization
    IFN-alpha/beta R2 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.8 kDa. The protein migrates as 34-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IFN-alpha/beta R2 SDS-PAGE

Human IFNAR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
IFN-alpha/beta R2 MALS images

The purity of Human IFNAR2, His Tag (Cat. No. IF2-H5224) is more than 85% and the molecular weight of this protein is around 32-42 kDa verified by SEC-MALS.

Bioactivity-SPR
 IFN-alpha/beta R2 SPR

Human IFN-alpha 2b Protein, Fc Tag (Cat. No. IFB-H5253) immobilized on CM5 Chip can bind Human IFNAR2, His Tag (Cat. No. IF2-H5224) with an affinity constant of 0.257 μM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 IFN-alpha/beta R2 BLI

Loaded Human IFNAR2, His Tag (Cat. No. IF2-H5224) on HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) with an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    Interferon alpha/beta receptor 2 (IFNAR2) is also known as IFN-alpha binding protein, IFN-alpha/beta receptor 2, Type I interferon receptor 2, IFNABR and IFNARB, which is a single-pass type I membrane protein and belongs to the type II cytokine receptor family. IFNAR2 can associate with IFNAR1 to form the type I interferon receptor. IFNAR2 is a receptor for interferons alpha and beta.IFNAR2 involves in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 of IFNAR2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 of IFNAR2 is a potent inhibitor of type I IFN receptor activity.Genetic variations in IFNAR2 influence susceptibility to hepatitis B virus (HBV) infection.
  • Clinical and Translational Updates

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