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Human Glutaredoxin 1 / GLRX1 Protein, His Tag

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  • Synonym
    GLRX,GRX,TTase-1,GLRX1,GRX1
  • Source
    Human Glutaredoxin 1, His Tag(GLX-H5149) is expressed from E. coli cells. It contains AA Met 1 - Gln 106 (Accession # NP_001112362).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Glutaredoxin 1 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 12.7 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glutaredoxin 1 SDS-PAGE

Human Glutaredoxin 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    Glutaredoxin-1 (GRX1) is also known as Thioltransferase-1 (TTase-1), GLRX, GRX, GLRX1, which belongs to the glutaredoxin family. GRX1 / GLRX contains one glutaredoxin domain, which exists in either a reduced or an oxidized form. GRX1 / GLRX has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase and functions as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.
  • Clinical and Translational Updates

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