Immune Checkpoint Proteins

Figure 1. The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) is more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

The consistency in purity and bioactivity is essential for recombinant proteins.All our proteins have to pass a variety of purity and bioactivity assessments.

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)（Cat. No. PD1-H82E4）

PD-1&PD-L1 pathway

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind anti-PD-1 mab with a linear range of 0.1-3 ng/mL (Routinely tested).            

PD-1&PD-L1 pathway

Serial dilutions of anti-PD-1 mab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

LAG-3 pathway

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

LAG-3 pathway

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

TIGIT pathway

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

CD47&SIPR α pathway(Cell-based assay)

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

Cell-based assay

4-1BB pathway

Human 4-1BB Ligand (71-254) Protein, Fc Tag, premium grade (Cat. No. 41L-H5269) induce IL‐8 secretion in HT1080 human CD137 cell line. The specific activity of Human 4-1BB Ligand (71-254) Protein, Fc Tag, premium grade is＞1.00*10^5 U/mg (QC tested).

Pharmacokinetics (PK) analysis

CTLA-4 pathway

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No. CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag, His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).