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Rituximab

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RIB-Y35c Mouse Anti-Rituximab Antibody (MALS verified)
RIB-Y35c-sds
RIB-FY35c Mouse FITC-Labeled Anti-Rituximab Antibody, Mouse IgG1
RIB-FY35c-sds
RIB-Y36 Mouse Anti-Rituximab Antibody (AY36) (recommended for ADA assay)
RIB-Y36-sds
RIB-Y37 Mouse Anti-Rituximab Antibody (AY37) (recommended for PK/PD)
RIB-Y37-sds
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Part of Bioactivity data

RIB-FY35c-Cell-based assay
Rituximab FACS

2e5 of Anti-CD20 (Rituximab) CAR-293 cells were stained with 100 μL of 3 μg/mL of FITC-Labeled Anti-Rituximab Antibody, Mouse IgG1 (Cat. No. RIB-FY35c) and isotype control antibody respectively. FITC signal was used to evaluate the binding activity (QC tested).

Protocol
RIB-Y36-Cell-based assay
Rituximab FACS

FACS analysis shows that the binding of rituximab to 293F overexpressing CD20 was inhibited by increasing concentration of Anti-Rituximab Antibody (AY36) (Cat. No. RIB-Y36). The concentration of rituximab used is 10 ng/ml. The IC50 is 0.025 μg/ml (Routinely tested).

Protocol
RIB-Y36-SPR
Rituximab SPR

Anti-Rituximab Antibody (AY36) (mouse IgG1, Cat. No. RIB-Y36) captured on CM5 chip via anti-mouse antibodies surface, can bind human rituximab with an affinity constant of 0.01 nM.

Protocol
RIB-FY35c-ELISA
Rituximab ELISA

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Anti-Rituximab Antibody, Mouse IgG1 (Cat. No. RIB-FY35c) with a linear range of 0.01-0.3 μg/mL (QC tested).

Protocol

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Background

Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids

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