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> Star Ribbon Pre-stained Protein Marker
| Cat. No. | Product Description | Size/Price |
|---|
Clear separation between bands for a more precise MW identification.
Pre-stained protein markers in orange, blue and green for better MW visualization.
Our economical universal tools to saves your budget.
3 years shelf life when stored at -20°C.
1. Thaw the product at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
3. Load the following volumes of the product into each well for SDS-PAGE:
– 3-5 μL per well for mini gels
– 5-10 μL per well for large gels
*For Western blot experiments, the same volumes are recommended as above. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. For 1.5mm thick gels, the loading volume should be doubled.
1. This product has been prepared in SDS-PAGE loading buffer and can be used directly without boiling, diluting and adding reducing agent.
2. Longer transfer times or higher transfer voltages may be required for Western blot of large (>100 kDa) proteins.
3. Do NOT add SDS to the transfer buffer. If SDS must be used, the concentration should not exceed 0.02-0.04% for best performance.
4. In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.
5. Pre-stained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are still suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.
6. For your safety and health, please wear a lab coat and disposable gloves when using our product.
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