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TIGIT-Signalachse
TIGIT (T-Zellen-Immunrezeptor mit Ig und ITIM Domänen) ist ein neu entdecktes Immun-Checkpoint-Protein, das auf T-Zellen und natürlichen Killerzellen (NK) exprimiert wird. TIGIT ist ein hemmender Rezeptor, der die Immunaktivität moduliert, indem er mit dem aktivierenden Rezeptor CD226 (auch bekannt als DNAM-1) um die Ligandenbindung konkurriert. Dieses An- und Ausschalten von TIGIT und DNAM-1 verläuft parallel zu dem von CTLA4 & CD28, was sie zu einem vielversprechenden Ziel für die Anti-Tumor-Therapie macht.
CD155 (PVR) und CD112 (PVRL2 oder Nectin-2) sind Co-Liganden von TIGIT und DNAM-1, während CD113 (PVRL3 oder Nectin-3) nur an TIGIT bindet. Die Affinität zwischen verschiedenen Liganden und Rezeptoren ist unterschiedlich. Die Bindung zwischen TIGIT und CD155 ist stärker als die zwischen TIGIT und CD112 oder CD113.
ACROBiosystems führt eine große Sammlung rekombinanter Proteine der TIGIT-Signalachse. Neben den häufig verwendeten his- und Fc-markierten Proteinen führen wir auch mehrere vorbiotinylierte Proteine, wie z. B. biotinyliertes Human TIGIT, Avi-Tag (Avitag™) (Kat. Nr. TIT-H82E5), biotinyliertes Human CD155 / PVR-Protein, Fc-Tag, Avi-Tag (Avitag™) (Kat. Nr. CD5-H82F6) und biotinyliertes Human-Nectin-2 / CD112-Protein, Fc-Tag, Avi-Tag (Avitag™) (Kat. Nr. CD2-H82F8). Im Folgenden finden Sie ausführliche Informationen:
Fig.1 FACS assay shows Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT is 0.03 μg/mL.
Fig.2 FACS assay shows that Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/mL.
Fig.3 Anti-Human TIGIT MAb (Mouse IgG1) captured on CM5 chip via Anti-Mouse antibodies surface, can bind Human TIGIT, His Tag (Cat. No. TIT-H52H3) with an affinity constant of 3.93 nM as determined in a SPR assay (Biacore T200).
Fig.4 Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, His Tag (Cat. No. TIT-H52H3): Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.
Cat. No. | Species | Product Description | Structure | Purity | Features |
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TIT-H82E5 (Biotin-labeled) |
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Biotinylated Human TIGIT Protein, Avi Tag |
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TIT-H82F1 (Biotin-labeled) |
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Biotinylated Human TIGIT Protein, Fc Tag, Avi Tag |
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TIT-C5223 |
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Cynomolgus / Rhesus macaque TIGIT Protein |
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TIT-C5254 |
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Cynomolgus / Rhesus macaque TIGIT Protein, Fc Tag |
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TIT-M52E6 |
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Mouse TIGIT Protein, His Tag |
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TIT-M5257 |
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Mouse TIGIT Protein, Fc Tag |
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TIT-R5258 |
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Rat TIGIT Protein, Fc Tag |
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TIT-R5259 |
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Rabbit TIGIT Protein, Fc Tag |
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TIT-H52H3 |
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Human TIGIT Protein, His Tag |
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TIT-H5253 |
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Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin |
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TIT-H5254 |
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Human TIGIT Protein, Fc Tag |
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TIT-C52H7 |
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Canine TIGIT Protein, His Tag |
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Molecule | Cat. No. | Species | Product Description | Structure | Purity | Features |
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CD155 | CD5-H82F6 |
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Biotinylated Human CD155 / PVR Protein, Fc, Avi Tag |
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CD5-R52H5 |
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Rhesus macaque CD155 / PVR Protein, His Tag |
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CD5-H5254 |
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Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin |
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CD5-M5254 |
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Mouse CD155 / PVR Protein, Mouse IgG2a Fc Tag |
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CD5-H82E3 |
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Biotinylated Human CD155 / PVR Protein, His, Avitag™ |
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CD5-M82F7 |
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Biotinylated Mouse CD155 / PVR Protein, Fc, Avitag™ |
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CD5-H5251 |
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Human CD155 / PVR Protein, Fc Tag |
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CD5-H5223 |
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Human CD155 / PVR Protein, His Tag |
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CD112 | CD2-H82F8 |
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Biotinylated Human Nectin-2 / CD112 Protein, Fc, Avi Tag |
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CD2-H5257 |
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Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc Tag |
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PV2-H5253 |
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Human Nectin-2 / CD112 Protein, Fc Tag |
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PV2-H52E2 |
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Human Nectin-2 / CD112 Protein, His Tag |
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CD113 | PV3-H82F3 |
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Biotinylated Human Nectin-3 / CD113 Protein, Fc, Avi Tag |
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PV3-H5255 |
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Human Nectin-3 / CD113 Protein, Fc Tag |
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PV3-H52E4 |
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Human Nectin-3 / CD113 Protein, His Tag |
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DNAM-1 | DN1-H82F9 |
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Biotinylated Human DNAM-1 / CD226 Protein, Fc, Avi Tag |
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DN1-C52H9 |
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Cynomolgus DNAM-1 / CD226 Protein, His Tag |
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DN1-H5257 |
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Human DNAM-1 / CD226 Protein, Fc Tag |
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DN1-H52H6 |
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Human DNAM-1 / CD226 Protein, His Tag |
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