Save Product
Product Details
Source
Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
16H5
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Human
Immunogen
Human TNF-alpha Protein is expressed from human 293 cells
Conjugate
Biotin
Specificity
This product is a specific antibody specifically reacts with TNF-alpha.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
Customers Also Viewed
Performance Data
Bioactivity-ELISA
Immobilized Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) at 2 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) with a linear range of 0.20-3.13 ng/mL (QC tested).
Protocol
Immobilized Monoclonal Anti-TNF-alpha Antibody, Human IgG1 (13B8) (Cat. No. TNA-AM493) at 2 μg/mL, add increasing concentrations of Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228), and then add Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) at 0.25 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.02 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 75.9 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) on Protein A Biosensor, can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 2.13 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) on Protein A Biosensor, can bind Human TNF-alpha, premium grade (Cat. No. TNA-H4211) with an affinity constant of 3.16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor α
- Category：
- Approved Drugs：
68 Details
- Drugs in Clinical Trials：
74 Details
- Highest Development Stage：
Approved
Contact Us
Drug Candidate Licensing
