resDetect™ RNase Activity Assay Kit (Fluorescence)

Add 90 μL RNase Substrate Working Solution (mix RNase Substrate, 10×Reaction Buffer and Nuclease-free Water by 1:1:7 volume) to each 96-well plate, and add 10 μL RNase A standards (0-200 pg/mL×10 μL / well = 0-2 pg/well), incubate the plate in the fluorometer (BMG CLARIOstar) collecting real-time data at one minute intervals for 30 minutes at 37°C using the settings described in this section. The RNase Activity Assay can be evaluated in rigorous kinetic terms using real-time data.

This assay kit employs a standard detection of RNase A. Add 90 μL RNase Substrate Working Solution (mix RNase Substrate, 10×Reaction Buffer and Nuclease-free Water by 1:1:7 volume) to each 96-well plate, and add 10 μL of RNase A standards (0-200 pg/mL×10 μL/well = 0-2 pg/well), incubate for 30 minutes at 37°C. Then measure the fluorescence using the settings described in this section in a fluorometer (BMG CLARIOstar). Take RFU of standards as the ordinate and RNase concentration as the abscissa. Four parameters logistic are used to draw the standard curve. This following data is for reference only.(QC tested)

Eight replicates of each of seven samples containing different concentrations of RNase were tested in one assay, Intra-Assay Precision CV＜10%.

Eight replicates of each of seven samples containing different concentrations of RNase were tested in eight independent assays, Inter-Assay Precision CV

The probe is freeze-thawed 3 times, the kit performance meets the requirements, the sensitivity is not reduced, and the CV

Three different concentrations of RNase was spiked into four different kinds of matrixes. The range of the recovery rate is 80%~120%.

DNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A002) and RNase Activity Assay Kit (Fluorescence) (Cat. No. ASE-A001) were used to detect nuclease residues in the same sample. No significant cross-reactivity or interference was observed.