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Human Laminin Quantitative ELISA Kit

COADSMS
DS

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RES-A091-96tests
$510.00
ETA of in-stock products:2 business days
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Product Details

  • Product Specifications

    Assay Type
    Sandwich-ELISA
    Analyte
    Laminin
    Format
    96T (8×12 strips)
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    ＜3.125 ng/mL
    Standard Curve Range
    3.125 ng/mL-100 ng/mL
    Assay Time
    3 hr 20 min
    Suitable Sample Type
    For the quantitative determination of Laminin in human serum and cell culture supernates.
    Sample volume
    100 μL

  • Product Overview

    Human Laminin Quantitative ELISA Kit is based on the Sandwich-ELISA method and is used to detect and quantitatively measure Laminin, including the specific identification of full-length Laminin 521, Laminin 521 E8 fragment, full-length Laminin 511, and Laminin 511 E8 fragment levels, in cell culture supernatants, serum, and plasma. It is designed to provide rigorous quality control, helping to monitor Laminin levels in biological samples and contribute to process optimization in the production of cell-based therapies.

  • Reconstitution

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • Storage

    1. Store the unopened kit at 2-8°C upon receipt.

    2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.

  • Materials Provided

    ID
    Components
    Size
    RES091-C01
    Pre-coated Anti-Laminin Antibody Microplate
    1 plate (8×12 strips)
    RES091-C02
    Human Laminin 521 Standard
    20 μg
    RES091-C03
    Biotin-Anti-Laminin Antibody
    20 μg
    RES091-C04
    Streptavidin-HRP
    50 μL
    RES091-C05
    10×Washing Buffer
    50 mL
    RES091-C06
    2×Dilution Buffer
    50 mL
    RES091-C07
    Substrate Solution
    12 mL
    RES091-C08
    Stop Solution
    7 mL

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Typical Data 

    Please refer to DS document for the assay protocol.

    Laminin TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

  • Laminin TYPICAL DATA

    Human Laminin Quantitative ELISA Kit can recognize GMP Human Laminin 521 Protein (Cat. No. GMP-LA5H24), with a linear range of 3.125-100 ng/mL.

  • Laminin TYPICAL DATA

    Human Laminin Quantitative ELISA Kit can recognize GMP Human Laminin 511 Protein (Cat. No. GMP-LA1H25), with a linear range of 3.125-100 ng/mL.

Validation

  • Dilution Linearity

    To assess the linearity of the assay, samples spiked with high concentrations of Laminin 521 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

    Laminin DILUTION LINEARITY

  • Intra-Assay Statistics

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

    Laminin INTRA-ASSAY STATISTICS

  • Inter-Assay Statistics

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

    Laminin INTER-ASSAY STATISTICS

  • Recovery

    Three Laminin 521 with different concentrations were tested to calculate the recovery rate.

    Laminin RECOVERY

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