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Streptavidin(SA) Series Products

In 1963, Stapley EO first isolated streptavidin (SA) from the culture medium of Streptomyces avidinii. The binding between SA and biotin is one of the strongest non-covalent interactions known in nature. SA and biotin bind with high affinity producing a significant amplification effect, making the SA-biotin system an asset that is widely utilized in biological and chemical experiments.
SA-biotin systems are often coupled to antibodies or proteins, where SA is conjugated to labels such as HRP (Horseradish Peroxidase), fluorophores, etc., and biotin is coupled to antibodies or proteins (MABSOL® Biotinylated Proteins). The SA-biotin system is widely used in the qualitative and quantitative detection of trace antigens, antibodies and localization observation research to achieve indirect detection of targets, making immunolabeling and related tracer analysis more sensitive.
ACROBiosystem’s SA series of products includes recombinant SA protein, labeled SA protein, SA coated plates, SA magnetic beads, etc., to accelerate your scientific research projects and drug development. Among them, the labeled SA protein contains premium grade PE labeled SA protein, which are strictly sterile and can be used for preclinical research in cell-related experiments.

SA Series Products

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Verification Data

Application data for PE labeled SA protein(FACS)

Application data for PE labeled SA protein(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Application data for PE labeled SA protein(FACS)

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Application data for SA(ELISA)

STN-N5116-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

STN-N5116-E2

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Application data for SA-HRP

Application data for SA-HRP

Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.5-16 ng/mL when detected by Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NH913) (QC tested).

Application data for SA-HRP

Measure performance comparison between Acro (Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin) and other competitive products. With tight quality control measures, Acro demonstrates high sensitivity in this assay (as shown in the comparison data).

Application data for SA coated plate

SP-11-E1

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

SP-11-E2

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

SP-11-E3

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituxima* with a linear range of 78-625 ng/mL (QC tested).

SP-11-E4

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimuma* with a linear range of 78-1250 ng/mL (QC tested).

Application data for SA magnetic beads

SMB-B01-E1

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads （Cat. No. SMB-B01） can bind the Anti-SARS-CoV-S protein RBD Antibody with an EC50 1.157 μg/mL (QC tested).

Reference

  • Louis Chaiet, Frank J. Wolf. The properties of streptavidin, a biotin-binding protein produced by Streptomycetes, Archives of Biochemistry and Biophysics (1964). https://doi.org/10.1016/0003-9861(64)90150-X.

MABSOL® Biotinylierte Proteine

FAQ

Q

How do I choose the right streptavidin product for my application?

Choose the streptavidin format based on your assay workflow and detection method. Recombinant streptavidin proteins provide flexibility for general biotin-binding applications, while labeled streptavidin can be used for direct detection. Streptavidin-coated plates and magnetic beads provide ready-to-use solid-phase options for capture, immobilization, and separation workflows.
Q

Which labeled streptavidin should I choose for my detection platform?

Select the label according to your detection method and instrument. The ACROBiosystems portfolio includes HRP- and ALP-conjugated streptavidin for enzyme-based detection; PE, APC, FITC, Cy3, Cy5, Texas Red, and Alexa Fluor™ conjugates for fluorescence-based detection; and acridinium ester-labeled streptavidin for chemiluminescence-based applications.
Q

When should I use a streptavidin-coated plate instead of soluble streptavidin?

Streptavidin-coated plates are a convenient choice when biotinylated molecules need to be immobilized directly onto a solid surface for plate-based assays. Soluble streptavidin provides greater flexibility when the assay requires a customized coating, labeling, or detection workflow.
Q

Which streptavidin products are suitable for flow cytometry?

Fluorescently labeled streptavidin products can be used to detect biotinylated molecules in flow cytometry. For example, PE-labeled streptavidin has been validated in a FACS assay using biotinylated CD19 protein and anti-CD19 CAR-293 cells. Select the fluorophore according to your instrument configuration and multicolor panel design.
Q

Which streptavidin reagents are available for TR-FRET assays?

ACROBiosystems offers streptavidin reagents labeled with FA, Europium chelate, or Terbium chelate for TR-FRET applications. The appropriate reagent should be selected according to the donor-acceptor pair and overall TR-FRET assay design.
Q

How can streptavidin magnetic beads be used with biotinylated molecules?

Streptavidin magnetic beads provide a solid-phase platform for capturing and immobilizing biotinylated molecules through the streptavidin-biotin interaction. They can support magnetic separation and bead-based assay workflows, with different bead formats available for specific research and detection applications.
Q

What should I consider when using HRP-conjugated streptavidin in an ELISA?

When using HRP-conjugated streptavidin, optimize the reagent concentration and incubation conditions according to the amount of biotinylated target and the desired assay sensitivity. Appropriate blocking and washing conditions are also important for maintaining a good signal-to-background ratio. HRP-conjugated streptavidin products are available for biotin-based detection workflows.
Q

How can I reduce background in a streptavidin-biotin assay?

High background can be influenced by reagent concentration, insufficient blocking or washing, and non-specific interactions within the assay system. Optimize the concentrations of the biotinylated reagent and streptavidin conjugate, and ensure appropriate blocking and washing conditions. The optimal conditions should be determined for each specific assay format.
  • SA- Biotin Systems
  • SA Series Products
  • Verification Data
  • Reference
  • FAQ