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ELISA Solutions: High-Performance Buffers and Coated Plates

In ELISA assays, where precision and data reliability are critical, researchers often encounter challenges such as elevated background signals, suboptimal standard curve linearity, and complex assay workflows. These issues are frequently attributed to variations in buffer composition and the quality of key reagents. Even minor inconsistencies can substantially affect assay sensitivity, specificity, and reproducibility. To overcome these obstacles, we have developed a comprehensive range of ELISA buffers and coated plates, providing stable, high-performance solutions that ensure consistent results across both routine and specialized applications.

Buffers

In ELISA assays, critical steps such as blocking, washing, and sample dilution depend on robust and reliable buffers. Our optimized ELISA buffer effectively blocks non-specific binding sites on microplates, substantially reducing background signals and enhancing the assay’s signal-to-noise ratio. Each component is rigorously validated for compatibility to ensure seamless performance across all assay steps, supporting a broad dynamic range and excellent reproducibility. Furthermore, our dilution buffer is specifically formulated to minimize non-specific adsorption—including serum-induced background—thereby maximizing signal clarity and detection sensitivity.
Product List
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EBS-001 ELISA Buffer Set-96T

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EBS-002 ELISA Buffer Set (Phosphate system, 96T)

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DB-02 Dilution Buffer (Strengthen Blocking)

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Application Data
Validated by ELISA, the Dilution Buffer effectively reduces serum-derived background signals in SARS-CoV-2 Spike RBD detection

Validated by ELISA, the Dilution Buffer effectively reduces serum-derived background signals in SARS-CoV-2 Spike RBD detection

2% serum diluted by Dilution Buffer (Cat.No. DB-02 ): Biotinylated SARS-CoV-2 S protein RBD was put into, the background value is 2.383 and 2.148; 2% serum diluted by DB-02: Biotinylated SARS-CoV-2 S protein RBD was put into, the background value is 0.262 and 0.263. In the existence of DB-02, the serum background values significantly decreased. Note: Dilution Buffer is Wash Buffer with 0.5% (w/v) bovine serum albumin (BSA), 50 mL.

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Coated Plates

  • Streptavidin Coated Plates
  • Nickel-Coated Plates

Streptavidin Coated Plates

Designed for applications based on the biotin–streptavidin interaction, our Streptavidin-Coated Plates provide a convenient and reliable platform for assay development. The high-density streptavidin coating enables efficient and specific capture of biotinylated molecules while minimizing non-specific binding and background signals. By eliminating traditional plate-coating steps, these plates streamline workflow setup and improve experimental consistency. They offer a flexible solution for a wide range of applications, including ELISA, protein interaction analysis, and high-throughput target-binding studies.
Product List
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SP-11 Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame

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SP-13 Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing)

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SP-14 Streptavidin Coated Plates, Clear, 96-Well, Clear Frame

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SP-15 Streptavidin Coated Plates, Clear, 96-Well, Clear Frame (For Serological Testing)

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Application Data
Streptavidin coated plates: suitable for evaluating the binding activity between CD19 protein and anti-FMC63 antibody

Streptavidin coated plates: suitable for evaluating the binding activity between CD19 protein and anti-FMC63 antibody

Immobilized Biotinylated Human CD19 (20-291), His, Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (Routinely tested).

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Streptavidin coated plates: suitable for evaluating the binding activity between PD-1 and PD-L1

Streptavidin coated plates: suitable for evaluating the binding activity between PD-1 and PD-L1

Immobilized Biotinylated Canine PD-1, His&Avi tag at 1000ng/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well, Clear Frame (For Serological Testing) (Cat. No. SP-15), can bind PD-L1 with a linear range of 0.244-62.5ng/mL, EC50=2.27ng/mL (QC tested).


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Nickel-Coated Plates

In ELISA assays, critical steps such as blocking, washing, and sample dilution depend on robust and reliable buffers. Our optimized ELISA buffer effectively blocks non-specific binding sites on microplates, substantially reducing background signals and enhancing the assay’s signal-to-noise ratio. Each component is rigorously validated for compatibility to ensure seamless performance across all assay steps, supporting a broad dynamic range and excellent reproducibility. Furthermore, our dilution buffer is specifically formulated to minimize non-specific adsorption—including serum-induced background—thereby maximizing signal clarity and detection sensitivity.
Product List
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SP-19 Nickel Coated Plates, Clear, 12×8-Well Strips, White Frame

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Application Data
Nickel-coated plates: suitable for evaluating the binding activity between Siglec-10 protein and anti-Siglec-10 antibody

Nickel-coated plates: suitable for evaluating the binding activity between Siglec-10 protein and anti-Siglec-10 antibody

Immobilized Cynomolgus Siglec-10, His Tag at 1 μg/mL (100 μL/well) on Nickel Coated plates, Clear, 12×8-Well Strips (Cat.No. SP-19), can bind Anti-Cynomolgus Siglec-10 Antibody, Human IgG1 with a linear range of 0.005-0.313 μg/mL, CV% of Intra-assay tests＜10% (QC tested).

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Nickel-coated plates: suitable for assessing the binding activity between GLP1R protein and anti-GLP1R antibody

Nickel-coated plates: suitable for assessing the binding activity between GLP1R protein and anti-GLP1R antibody

Immobilized Human GLP1R Full Length Protein (L260F), Flag,His Tag at 5 μg/mL (100 μL/well) on Nickel Coated plates, Clear, 12×8-Well Strips (Cat.No. SP-19), the binding activity with Monoclonal Anti-GLP1R Antibody, Fc Tag (A6727) was the similar with the other nickel plate.

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