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Star Ribbon Pre-stained Protein Marker

Protein markers are an essential component in biological research and drug development. Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target protein or evaluate the transfer efficiency.
Star Ribbon Pre-stained Protein Marker is our newest member of ACROBiosystems' ComboX universal combination solutions. Star Ribbon products include pre-stained protein markers of different types including wide molecular weight range, low and high molecular weight. In each product, protein markers are pre-stained in three-colors. The indicator bands are accurate and reliable, bright and clear, and easy to observe.

Product List

Cat. No.MoleculeProduct DescriptionOrder Now
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Product Features

Clear separation between bands for a more precise MW identification.

Pre-stained protein markers in orange, blue and green for better MW visualization.

Our economical universal tools to save your budget.

3 years shelf life when stored at -20°C.

Product Display

Wide MW Range

Star Ribbon Pre-stained Protein Marker (8-180kDa)

（Cat. No. PPM-M1001）

PPM-M1001（8-180KDa）
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Low MW Range

Star Ribbon Pre-stained Protein Marker (2.7-40kDa)

（Cat. No. PPM-M1002）

PPM-M1002（2.7-40KDa）
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High MW Range

Star Ribbon Pre-stained Protein Marker (40-275kDa)

（Cat. No. PPM-M1003）

PPM-M1003（40-275KDa）
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Protocol

1. Thaw the product at room temperature for a few minutes to dissolve precipitated solids. Do not boil!

2. Mix gently, but thoroughly, to ensure the solution is homogeneous.

3. Load the following volumes of the product into each well for SDS-PAGE:
– 3-5 μL per well for mini gels
– 5-10 μL per well for large gels

*For Western blot experiments, the same volumes are recommended as above. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. For 1.5mm thick gels, the loading volume should be doubled.

Additional Notes

1. This product has been prepared in SDS-PAGE loading buffer and can be used directly without boiling, diluting and adding reducing agent.

2. Longer transfer times or higher transfer voltages may be required for Western blot of large (>100 kDa) proteins.

3. Do NOT add SDS to the transfer buffer. If SDS must be used, the concentration should not exceed 0.02-0.04% for best performance.

4. In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.

5. Pre-stained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are still suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.

6. For your safety and health, please wear a lab coat and disposable gloves when using our product.

FAQ

Q

How do I choose the right Star Ribbon Pre-stained Protein Marker for my target protein?

Choose the marker based on the expected molecular weight of your target protein. Star Ribbon Pre-stained Protein Markers are available in three ranges: 8–180 kDa (PPM-M1001) for a broad molecular weight range, 2.7–40 kDa (PPM-M1002) for low-molecular-weight proteins, and 40–275 kDa (PPM-M1003) for high-molecular-weight proteins.
Q

Can Star Ribbon Pre-stained Protein Markers be used for both SDS-PAGE and Western blotting?

Yes. Star Ribbon Pre-stained Protein Markers can be used in SDS-PAGE to estimate the approximate molecular weight of proteins and in Western blotting to monitor protein transfer. The pre-stained bands provide convenient visual reference throughout electrophoresis and transfer.
Q

Do I need to boil, dilute, or add reducing agents before loading the marker?

No. Star Ribbon Pre-stained Protein Markers are supplied in SDS-PAGE loading buffer and are ready to use without boiling, dilution, or the addition of reducing agents. Simply thaw the marker at room temperature for a few minutes and mix gently but thoroughly before loading.
Q

What loading volume is recommended for Star Ribbon Pre-stained Protein Markers?

For gels 0.75–1.0 mm thick, the recommended loading volume is 3–5 μL per well for mini gels and 5–10 μL per well for large gels. For 1.5 mm gels, doubling the loading volume is recommended. The same loading volumes can be used for Western blotting.
Q

Why can the apparent molecular weight of pre-stained protein bands vary between electrophoresis systems?

Pre-stained proteins may exhibit different electrophoretic mobilities depending on the SDS-PAGE buffer system and electrophoresis conditions. Therefore, pre-stained markers are best suited for approximate molecular weight estimation. When greater accuracy is required, migration should be calibrated against unstained protein standards under the same electrophoresis conditions.
Q

What should I consider when transferring high-molecular-weight proteins during Western blotting?

Large proteins, particularly those above 100 kDa, may require longer transfer times or higher transfer voltages for efficient transfer. Transfer conditions should be optimized according to the molecular weight of the target protein and the specific Western blot setup.
Q

Can SDS be added to the transfer buffer when using Star Ribbon Pre-stained Protein Markers?

Adding SDS to the transfer buffer is not recommended. If SDS is required for your experiment, keep the concentration within 0.02–0.04% for optimal marker performance.
Q

Why do low-molecular-weight marker bands sometimes migrate with the dye front?

In low-percentage gels (<10%), low-molecular-weight proteins in the marker may migrate with the dye front, making them more difficult to resolve. Selecting a gel percentage appropriate for the molecular weight range of interest can help improve band separation and visualization.
  • Introduction
  • Product List
  • Product Features
  • Product Display
  • FAQ