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Product Details
Synonyms
VEGFC, Flt4-L, VRP
Source
Human VEGF-C Protein, His Tag (VEC-H52H3) is expressed from human 293 cells (HEK293). It contains AA Thr 103 - Arg 227 (Accession # AAH35212.1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 31 kDa. The protein migrates as 40-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human VEGF-C Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) with a linear range of 1-16 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) capture on NTA-Series S sensor chip can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with an affinity constant of 0.130 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) immobilized on CM5 Chip can bind Human Neuropilin-1, His Tag (Cat. No. NR1-H5228) with an affinity constant of 7.50 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human VEGF-C Protein, His Tag (Cat. No. VEC-H52H3) immobilized on CM5 Chip can bind Human Neuropilin-2, His Tag (Cat. No. NR2-H52H3) with an affinity constant of 8.36 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Vascular endothelial growth factor C
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 2 Clinical
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