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Product Details
Synonyms
TL1A, VEGI, TNFSF15
Source
Human TL1A, His Tag (TLA-H5243) is expressed from human 293 cells (HEK293). It contains AA Leu 72- Leu 251 (Accession # O95150-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 26 kDa and 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.001 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human TL1A, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TL1A, His Tag (Cat. No. TLA-H5243) is more than 85% and the molecular weight of this protein is around 75-95 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Monoclonal Anti-TL1A Antibody, Human IgG1 and 4 μg/mL (50 μL/well) Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a half maximal inhibitory concentration (lC50) of 1.976 μg/mL (Routinely tested).
Protocol
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 0.005-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 45.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-TL1A antibody captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 0.765 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human DcR3, Fc Tag (Cat. No. TNB-H5255) captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 0.269 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human TL1A, His Tag (Cat. No. TLA-H5243) captured on CM5 chip via anti-His antibody can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with an affinity constant of 1.15 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Response to human TL1A protein (FOLD).
The DR3 (TL1A receptor) (Luc) Jurkat Reporter Cell was stimulated with serial dilutions of human TL1A protein (Cat.No.TLA-H5243). The max induction fold was approximately 40.12.Protocol
User Reviews Publish Comment
S***e2025-09-13
LikesWorked all times I have used this product with very reproducible data! Also appreciated the very quick shipping.- A***e2025-04-10 LikesI used the TL1A in multiple experiments and the results were always consistent and reproducible. Different lots were used throughout a year long timeline and the results did not differ between lots. When shipping there was not ever any damaged packaging that could impact product quality. I have used Acrobiosystems in my professional career multiple times over multiple years and have always had success :) I will continue to use Acro as a trusted supplier.
- E***r2024-08-08 LikesThis protein showed good activity in cell reporter assays and could be used routinely in inhibition assays.
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor superfamily member 15
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
18 Details
- Highest R&D Stage：
Phase 3 Clinical
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Drug Candidate Licensing
