Streptavidin Protein-HRP, ultra sensitivity

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Cat. No. / Size
Price
Qty
STN-NH113-100ug
$65.00
STN-NH113-1mg (500ug X 2)
$255.00
STN-NH113-5mg (500ug X 10)
$950.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Streptavidin, SA

  • Source

    Streptavidin Protein-HRP, ultra sensitivity (STN-NH113) is expressed from E. coli cells.

    Predicted N-terminus: Met

  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 13.8 kDa.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Streptavidin ELISA

    Immobilized Cynomolgus / Rhesus macaque BCMA, His Tag (Cat. No. BCA-C52H7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human APRIL, Fc,Avitag (Cat. No. APL-H82F5) with a linear range of 0.05-13 ng/mL when detected by Streptavidin Protein-HRP, ultra sensitivity (0.05 µg/mL) (Cat. No. STN-NH113) (QC tested).

    Protocol
  •  Streptavidin ELISA

    Measure performance comparison between Acro (Streptavidin Protein-HRP, ultra sensitivity) and other competitive products. With tight quality control measures, Acro has better sensitivity than other brands.

  •  Streptavidin ELISA

    Immobilized Monoclonal Mouse Anti-CD5-2C8A7, Mouse IgG1 at 1 μg/mL, add Human CD5, His Tag (Cat. No. CD5-H52H5) and then add Monoclonal Biotin-Mouse Anti-CD5-9F8F11, Mouse IgG1 at 0.5 μg/mL. Detection was performed using Streptavidin Protein-HRP, ultra sensitivity (Acro, Cat. No. STN-NH113) (Routinely tested).

    Protocol

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Background

Streptavidin is a 66 kDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44 kDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.

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  • Performance Data
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