Streptavidin Protein-Cy3

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Cat. No. / Size
Price
Qty
STN-NC114-100ug
$55.00
STN-NC114-1mg (200ug X 5)
$165.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Streptavidin, SA

  • Source

    Streptavidin Protein-Cy3 (STN-NC114) is expressed from E. coli cells.

    Predicted N-terminus: Met

  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 13.8 kDa.

  • Conjugate

    Cy3

    Excitation Wavelength: 555 nm

    Emission Wavelength: 569 nm

  • Labeling

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with Cy3 using standard chemical labeling method. The residual Cy3 is removed by molecular sieve treatment during purification process.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     Streptavidin FACS

    5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-CY3 (Cat. No. STN-NC114) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.

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