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Human SIRP beta / CD172b Protein, Fc Tag (MALS verified)

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Product Number/Spec
Price
Menge
SIA-H5257-100ug
$360.00
SIA-H5257-1mg (500ug X 2)
$2615.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    CD172b antigen, CD172b, DKFZp686A05192, FLJ26614, SIRP beta 1, SIRPB1, SIRP-BETA-1, 9930027N05Rik, SIRP-beta

  • Source

    Human SIRP beta, Fc Tag (SIA-H5257) is expressed from human 293 cells (HEK293). It contains AA Glu 30 - Leu 371 (Accession # O00241-1).

    Predicted N-terminus: Glu 30

    Request for sequence
  • Molecular Characterization

    SIRP beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 63.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    SIRP beta SDS-PAGE

    Human SIRP beta, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    SIRP beta SEC-MALS

    The purity of Human SIRP beta, Fc Tag (Cat. No. SIA-H5257) is more than 95% and the molecular weight of this protein is around 130-158 kDa verified by SEC-MALS.

    Report

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Publish Comment

Background Introduction

Signal-regulatory protein beta-1 (SIRP beta 1) also known as SIRPβ1, belongs to signal-regulatory-protein (SIRP) family, and immunoglobulin superfamily. SIRPβ1 is a transmembrane protein that has three Ig-like domains in its extracellular region and a short cytoplasmic tail. Human SIRPβ1 is expressed on monocytes and granulocytes but not on lymphocytes. SIRP beta 1 contains short cytoplasmic domains that lack cytoplasmic sequence motifs capable of recruiting SHP-2 and SHP-1. In addition, they contain a single basic lysine residue within the hydrophobic transmembrane domain.

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