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Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Rat SIRP alpha, Fc Tag (SIA-R5255) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # BAA20368.1).
Predicted N-terminus: Lys 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 64.0 kDa. The protein migrates as 100-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Rat SIRP alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Rat SIRP alpha, Fc Tag (Cat. No. SIA-R5255) is more than 85% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Rat SIRP alpha, Fc Tag (Cat. No. SIA-R5255) at 5 μg/mL (100 μL/well) can bind Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) at 5 μg/mL (100 μL/well) can bind Rat SIRP alpha, Fc Tag (Cat. No. SIA-R5255) with a linear range of 2-39 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
19 Details
- Highest R&D Stage：
Approved
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