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Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Mouse SIRP alpha, His Tag (SIA-M52H4) is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # BAA13520.1).
Predicted N-terminus: Lys 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.8 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Mouse SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Mouse SIRP alpha, His Tag (Cat. No. SIA-M52H4) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M525a) at 2 μg/mL (100 μL/well) can bind Mouse SIRP alpha, His Tag (Cat. No. SIA-M52H4) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Mouse SIRP alpha, His Tag (Cat. No. SIA-M52H4) immobilized on CM5 Chip can bind Mouse CD47, His Tag (Cat. No. CD7-M522b) with an affinity constant of 2.52 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
19 Details
- Highest R&D Stage：
Approved
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