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Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) is produced via site-specific conjugation of PE to Human SIRP alpha, Fc Tag under optimal conditions with a proprietary technology. Human SIRP alpha, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # P78324-1).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.7 kDa.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
Flow Cytometry (Neutralizing assay), Please note that this product is NOT compatible to streptavidin detection system.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
Bioactivity-FACS
Flow Cytometry assay shows that PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) can bind to Jurkat cells overexpressing human CD47. 1 μL stock solution per million cells (QC tested).
Protocol
FACS analysis shows that the binding of PE-Labeled Human SIRP alpha, Fc Tag (Cat. No. SIA-HP252) to Jurkat cells overexpressing CD47 was inhibited by increasing concentration of neutralizing anti-human CD47 antibody. The IC50 is 0.37 μg/mL (QC tested).
Protocol
User Reviews Publish Comment
R***n2024-06-03
LikesI recently used the PE-Labeled Human SIRP alpha / CD172a Protein, Fc Tag for detecting the expression of CD47 in Jurkat T-cells and primary T-cells, and I am very pleased with the results. The SIRP alpha reagent effectively binds to CD47 overexpressed in both Jurkat and primary T-cells. This experiment was conducted to demonstrate the overexpression of CD47 in T-cells, using the anti-CD47 antibody (clone: CC2C6) to confirm the expression. Additionally, it was crucial to show that our CD47 can bind to its natural ligand, SIRP alpha. The reagent performed excellently in this regard, providing clear and reliable results. I've attached an image showing the data with a CD47 KO Jurkat cell line, further validating the specificity and effectiveness of this reagent. Overall, I highly recommend this product for CD47 detection and binding studies.
- J***r2022-08-18 LikesI used this SIRPa-PE Fc tag for a FACS assay using the recommended SOP provided by Acro and our rep Nik Kamachi. Nik was very helpful in providing insight on the capabilities of Acro to provide custom labeled SIRPa Fc tags to meet our needs for small molecule screening assays that are FACS and IF-based. The human SIRPa-PE Fc tag was very easy to use and gave a strong signal with both flow cytometry and imaging
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
19 Details
- Highest R&D Stage：
Approved
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Drug Candidate Licensing
