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Product Details
Synonyms
SHPS1, SIRPA, CD172A, BIT, MFR, MYD1, P84, PTPNS1
Source
Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (SIA-C82F3) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 370 (Accession # NP_001271679.1).
Predicted N-terminus: Glu 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 65.3 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-C82F3) is more than 95% and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag (Cat. No. CD7-C5252) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-C82F3) with a linear range of 1-78 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus / Rhesus macaque CD47, His Tag (Cat. No. CD7-C52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus SIRP alpha Protein, Fc,Avitag (Cat. No. SIA-C82F3) with a linear range of 0.005-0.313 μg/mL (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Signal-regulatory protein alpha
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
19 Details
- Highest R&D Stage：
Approved
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