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Product Details
Synonyms
BSRPC
Source
Human SEZ6 Protein, Mouse IgG1 Fc Tag (SE6-H52F3) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - His 925 (Accession # Q53EL9-1).
Predicted N-terminus: Leu 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 123.9 kDa. The protein migrates as 200-235 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human SEZ6 Protein, Mouse IgG1 Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human SEZ6 Protein, Mouse IgG1 Fc Tag (Cat. No. SE6-H52F3) was greater than 90% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human SEZ6 Protein, Mouse IgG1 Fc Tag (Cat. No. SE6-H52F3) at 1 μg/mL (100 μL/well) can bind Anti-SEZ6 Antibody (SC17) with a linear range of 0.06-4 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-SEZ6 Antibody (SC17) captured on CM5 chip via anti-Human IgG Fc antibody can bind Human SEZ6 Protein, Mouse IgG1 Fc Tag (Cat. No. SE6-H52F3) with an affinity constant of 6.33 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Seizure protein 6 homolog
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 3 Clinical
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