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Product Details
Synonyms
Polymerase acidic protein
Source
Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag (PON-V51H3) is expressed from E. coli cells. It contains AA Met 1 - Gly 209 (Accession # P31343-1).
Predicted N-terminus: Met 1
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, Glycerol, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag (Cat. No. PON-V51H3) at 1 μg/mL (100 μL/well) can bind Influenza A PA Polyclonal Antibody,Rabbit/IgG with a linear range of 0.2-16 ng/mL (QC tested).
Protocol
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