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Product Details
Synonyms
OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1, CD134 ligand, CD134L
Source
Mouse OX40 Ligand Protein, Fc Tag (OXL-M5263) is expressed from human 293 cells (HEK293). It contains AA Ser 51 - Leu 198 (Accession # P43488-1).
Predicted N-terminus: Pro
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 43.1 kDa. The protein migrates as 43-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse OX40 Ligand Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse OX40 Ligand Protein, Fc Tag (Cat. No. OXL-M5263) is more than 95% and the molecular weight of this protein is around 258-295 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse OX40 Ligand Protein, Fc Tag (Cat. No. OXL-M5263) at 2 μg/mL (100 μL/well) can bind Mouse OX40, His Tag (Cat. No. OX0-M5228) with a linear range of 2-39 ng/mL (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 4
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
13 Details
- Highest R&D Stage：
Phase 3 Clinical
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