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Product Details
Synonyms
OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1, CD134 ligand, CD134L
Source
Mouse OX40 Ligand Protein, His Tag (OXL-M5243) is expressed from human 293 cells (HEK293). It contains AA Ser 51 - Leu 198 (Accession # P43488-1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 53.3 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse OX40 Ligand Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse OX40 Ligand Protein, His Tag (Cat. No. OXL-M5243) is more than 95% and the molecular weight of this protein is around 48-68 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse OX40, Fc Tag (Cat. No. OX0-M5259) at 10 μg/mL (100 μL/well) can bind Mouse OX40 Ligand Protein, His Tag (Cat. No. OXL-M5243) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Bioactivity-CELL BASE
Mouse OX40 Ligand Protein, His Tag (Cat. No. OXL-M5243) induced OX40 (Luc) reporter activity in HEK293 cells. The EC50 for this effect is 0.070 µg/mL.
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 4
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
13 Details
- Highest Development Stage：
Phase 3 Clinical
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