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Product Details
Synonyms
OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1, CD134 ligand, CD134L
Source
Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag (OXL-C5243) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # A0A1D5QQH7 ). In the region Gln 51 - Leu 183, the amino acid sequences of Cynomolgus and Rhesus macaque OX40 Ligand are identical.
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 49.3 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus / Rhesus macaque OX40 Ligand Protein, His Tag (Cat. No. OXL-C5243) is more than 95% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag (Cat. No. OXL-C5243) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque OX40, Fc Tag (Cat. No. OX0-C5251) with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus/Rhesus macaque OX40 Ligand, His Tag (Cat. No. OXL-C5243) at 2 μg/mL (100 μL/well) can bind Human OX40, Fc Tag (Cat. No. OX0-H5255) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus / Rhesus macaque OX40, Fc Tag (Cat. No. OX0-C5251) at 5 μg/mL (100 μL/well) can bind Cynomolgus/Rhesus macaque OX40 Ligand, His Tag (Cat. No. OXL-C5243) with a linear range of 20-78 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 4
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
13 Details
- Highest R&D Stage：
Phase 3 Clinical
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