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Product Details
Synonyms
NKG2A & CD94
Source
Human NKG2A&CD94, Fc Tag (NC4-H5257) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # P26715-1 (NKG2A) & Q13241-1 (CD94)).
Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 55.1 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human NKG2A&CD94, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human NKG2A&CD94, Fc Tag (Cat. No. NC4-H5257) is more than 95% and the molecular weight of this protein is around 125-145 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human NKG2A&CD94 Protein, Fc Tag (Cat. No. NC4-H5257) at 2 μg/mL (100 μL/well) can bind Anti-NKG2A Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
NK cell lectin-like receptor subfamily C member 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
6 Details
- Highest R&D Stage：
Phase 3 Clinical
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