FITC-Labeled Human LILRB4 / CD85k / ILT3 Protein, His Tag

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Cat. No. / Size
Price
Qty
LI4-HF2H3-25ug
$330.00
LI4-HF2H3-200ug
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    LILRB4, ILT3, LIR5, CD85K, HM18

  • Source

    FITC-Labeled Human LILRB4, His Tag (LI4-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1). It is the FITC labeled form of Human LILRB4, His Tag (Cat. No. LI4-H52H7).

    Predicted N-terminus: Gln 22

    Request for sequence
  • Molecular Characterization

    LILRB4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • Protein Ratio

    The FITC to protein molar ratio is 2-4.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    LILRB4 SDS-PAGE

    FITC-Labeled Human LILRB4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     LILRB4 ELISA

    Immobilized Anti-LILRB4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human LILRB4, His Tag (Cat. No. LI4-HF2H3) with a linear range of 0.02-0.625 μg/mL (QC tested).

    Protocol

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Background

Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

Recent Advances

 
Drug Development Progress
  • English Name:

    Leukocyte immunoglobulin-like receptor subfamily B member 4

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    7 Details

  • Highest Development Stage:

    Phase 2 Clinical

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