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Product Details
Synonyms
LILRB4, ILT3, LIR5, CD85K, HM18
Source
Human LILRB4, His Tag (LI4-H52H7) is expressed from human 293 cells (HEK293).
Predicted N-terminus: Gln 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human LILRB4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human LILRB4, His Tag (Cat. No. LI4-H52H7) is more than 95% and the molecular weight of this protein is around 28-41 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human LILRB4, His Tag (Cat. No. LI4-H52H7) at 1 μg/mL (100 μL/well) can bind Anti-LILRB4 Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human ANGPTL7, His Tag immobilized on CM5 Chip can bind Human LILRB4, His Tag (Cat. No. LI4-H52H7) with an affinity constant of 4.67 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Leukocyte immunoglobulin-like receptor subfamily B member 4
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
7 Details
- Highest R&D Stage：
Phase 2 Clinical
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