Cynomolgus Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein, His Tag&Tag Free, low endotoxin

Synonyms

Integrin alpha X beta 2, ITGAX&ITGB2, CD11C&CD18

Source

Cynomolgus ITGAX&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-C52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 20 -Pro 1107 (ITGAX) & Gln 23 - Asn 700 (ITGB2) (Accession # XP_015298151.3 (ITGAX) & XP_065397351.1 (ITGB2)).

Predicted N-terminus: Phe 20 (ITGAX) & Gln 23 (ITGB2)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Cynomolgus ITGAX&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAX and ITGB2, has a calculated MW of 125.9 kDa (ITGAX) and 78.0 kDa (ITGB2). Subunit ITGAX is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The protein migrates as 150-180 kDa (ITGAX) and 95-100 kDa (ITGB2) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process