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Product Details
Synonyms
Integrin alpha 2b beta 3, ITGAIIb&ITGB3
Source
Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free (IT3-H52W8) is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Arg 993 (ITGAIIb) & Gly 27 - Asp 718 (ITGB3) (Accession # P08514-1 (ITGAIIb) (R887L) & P05106-1 (ITGB3)).
Predicted N-terminus: Leu 32 (ITGAIIb) & Gly 27 (ITGB3)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAIIb and ITGB3, has a calculated MW of 111.4 kDa (ITGAIIb) and 81.8 kDa (ITGB3). Subunit ITGAIIb is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 125-130 kDa (ITGAIIb) and 90-95 kDa (ITGB3) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52W8) is more than 90% and the molecular weight of this protein is around 190-235 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Fibronectin at 2 μg/mL (100 μL/well) can bind Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52W8) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52W8) at 1 μg/mL (100 μL/well) can bind Abciximab with a linear range of 0.8-6 ng/mL (Routinely tested).
Protocol
Immobilized Human ITGAIIb&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52W8) at 5 μg/mL (100 μL/well) can bind Anti-DUSP2 Monoclonal Antibody (PAC-1) with a linear range of 0.5-31 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Integrin alpha-IIb/beta-3
- Category：
- Listed Drugs Count：
6 Details
- Clinical Drugs Count：
7 Details
- Highest R&D Stage：
Approved
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