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Product Details
Synonyms
IL-23 alpha & IL-12 beta
Source
Cynomolgus IL-23A&Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-CM52W8) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # A0A2K5TLV4-1 (IL23A) & P43432-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.3 kDa (IL23A) & 35.8 kDa (IL12B). The protein migrates as 20-23 kDa & 40-42 kDa and 43-46 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus IL-23A&Mouse IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus IL-23A&Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-CM52W8) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-CM52W8) with a linear range of 0.03-0.25 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on Protein A Biosensor, can bind Cynomolgus IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-CM52W8) with an affinity constant of 9.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-12 subunit beta
- Category：
- Listed Drugs Count：
7 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Approved
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