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Product Details
Synonyms
IL4, BCGF1, BSF1
Source
Human IL-4 Protein, Fc Tag (IL4-H5253) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).
Predicted N-terminus: His 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human IL-4 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) is more than 95% and the molecular weight of this protein is around 82-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-4 R alpha, His Tag (Cat. No. ILR-H5221) at 5 μg/mL (100 μL/well) can bind Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Immobilized Human IL-4 Protein, Fc Tag (Cat. No. IL4-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-4 R alpha, Avitag,His Tag (Cat. No. ILR-H82E9) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-4
- Category：
- Listed Drugs Count：
2 Details
- Clinical Drugs Count：
14 Details
- Highest R&D Stage：
Approved
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