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Product Details
Synonyms
IL2RA, CD25, p55, IL2-RA, IL-2-RA
Source
Human IL-2 R alpha, His Tag (ILA-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Cys 213 (Accession # NP_000408).
Predicted N-terminus: Glu 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.6 kDa. The protein migrates as 32-42 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human IL-2 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-2, Fc,Avitag (Cat. No. IL2-H82F3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 29.9 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 18 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) inhibits the IL-2 dependent proliferation of Mo7e cells. The EC50 for this effect is 0.35-0.77 µg/mL (Routinely tested).
Protocol
User Reviews Publish Comment
E***n2023-04-08
LikesYeast cells expressing and displaying different IL2 variants were incubated 1h in the presence of CD25 protein with His tag (Human Il-2 R alpha Acrobiosystems #ILA-H52H9) at a final concentration of 4µg/ml. Next, cells were washed and incubated with a mouse Anti-His-PE. The yeast cells were washed and were analyzed using FACS. We observed detectable amounts of fluorescence bound to yeast cells displaying specific IL2 variants confirming that CD25 could bind to certain constructs and providing evidence that certain IL2 variants were correctly folded.
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-2 receptor alpha chain
- Category：
- Listed Drugs Count：
3 Details
- Clinical Drugs Count：
16 Details
- Highest R&D Stage：
Approved
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