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Product Details
Synonyms
IL1RL1, DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1
Source
Human IL-1RL1, His Tag (IL1-H5229) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Phe 328 (Accession # Q01638-2).
Predicted N-terminus: Lys 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human IL-1RL1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IL-1RL1, His Tag (Cat. No. IL1-H5229) is more than 95% and the molecular weight of this protein is around 49-66 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-1RL1, His Tag (Cat. No. IL1-H5229) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-33, His,Avitag (Cat. No. IL3-H82H5) with a linear range of 0.02-1.25 μg/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-1 receptor-like 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
5 Details
- Highest R&D Stage：
NDA/BLA
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