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Product Details
Synonyms
IL13RA1, CD213A1, IL-13Ra, NR4, RP13-128O4.2, IL13Rα1
Source
Mouse IL-13RA1, Fc Tag (IL1-M5258) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Thr 340 (Accession # NP_598751).
Predicted N-terminus: Ala 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 62.7 kDa. The protein migrates as 67-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse IL-13RA1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) at 2 μg/mL (100 μL/well) can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Immobilized Mouse IL-13, His Tag (Cat. No. IL3-M5249) at 5 μg/mL (100 μL/well) can bind Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) with a linear range of 0.3-5 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) on Protein A Biosensor, can bind Human IL-13 Protein, His Tag, premium grade (Cat. No. IL3-H52H3) with an affinity constant of 17.2 nM as determined in BLI assay (ForteBio Octet R8) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-13 receptor subunit alpha-1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 2 Clinical
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