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Cynomolgus IL-13 Protein, His Tag, low endotoxin (MALS verified)

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Product Number/Spec
Price
Menge
IL3-C5249-50ug
$460.00
IL3-C5249-500ug
$2450.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13

  • Source

    Cynomolgus IL-13, His Tag (IL3-C5249) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Asn 132 (Accession # ABG75889.1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    IL-13 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 14.2 kDa. The protein migrates as 25-39 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    IL-13 SDS-PAGE

    Cynomolgus IL-13, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-13 SEC-MALS

    The purity of Cynomolgus IL-13, His Tag (Cat. No. IL3-C5249) is more than 95% and the molecular weight of this protein is around 22-30 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-13 ELISA

    Immobilized Cynomolgus IL-13, His Tag (Cat. No. IL3-C5249) at 5 μg/mL (100 μL/well) can bind Human IL-13RA2, Fc Tag (MALS verified) (Cat. No. IL2-H5256) with a linear range of 0.2-10 ng/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

Frontier Progress

 
Drug Development Progress
  • English Name:

    Interleukin-13

  • Category:

  • Listed Drugs Count:

    4 Details

  • Clinical Drugs Count:

    27 Details

  • Highest R&D Stage:

    Approved

Drug Candidate Licensing
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