Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (MALS & SPR verified)

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Cat. No. / Size
Price
Qty
HNN-M52H3-50ug
$465.00
HNN-M52H3-500ug
$2885.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Hemagglutinin-neuraminidase/HN (MuV)

  • Source

    Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (HNN-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 106 - Thr 582 (Accession # P11235).

    Predicted N-terminus: Gly 106

    Request for sequence
  • Molecular Characterization

    Hemagglutinin-neuraminidase/HN (MuV) Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 54.6 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Hemagglutinin-neuraminidase/HN (MuV) SDS-PAGE

    Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    Hemagglutinin-neuraminidase/HN (MuV) SEC-MALS

    The purity of Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) is more than 90% and the molecular weight of this protein is around 63-78 kDa verified by SEC-MALS.

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  • Bioactivity-SPR

     Hemagglutinin-neuraminidase/HN (MuV) SPR

    Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) immobilized on CM5 Chip can bind 3'-Sialyllactose / GM3 Ganglioside oligosaccharide with an affinity constant of 586 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Background

Mumps immunity is typically assessed by measuring neutralizing-antibody responses directed against mumps HN and F proteins.

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