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Product Details
Synonyms
FcRn, FCGRT & B2M
Source
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (FCM-C82W3) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1 (FCGRT) & Q8SPW0-1 (B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the amino acid sequences of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are identical.
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag, produced by co-expression of FCGRT and B2M, has a calculated MW of 34.1 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus , followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-40 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (Cat. No. FCM-C82W3) is more than 90% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (Cat. No. FCM-C82W3) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin with an affinity constant of 0.28 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus / Rhesus macaque FcRn Heterodimer Protein, His,Avitag (Cat. No. FCM-C82W3) on SA Biosensor, can bind Cynomolgus Serum Albumin, His Tag (Cat. No. CSA-C52H4) with an affinity constant of 0.617 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
IgG receptor FcRn large subunit p51
- Category：
- Listed Drugs Count：
4 Details
- Clinical Drugs Count：
5 Details
- Highest R&D Stage：
Approved
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