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Product Details
Synonyms
FAP, FAPalpha, SIMP, Seprase, APCE
Source
Human FAP, Fc Tag (FAP-H5263) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).
Predicted N-terminus: Pro
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 111.5 kDa. The protein migrates as 120-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human FAP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human FAP, Fc Tag (Cat. No. FAP-H5263) is more than 95% and the molecular weight of this protein is around 216-264 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human FAP, Fc Tag (Cat. No. FAP-H5263) at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Anti-FAP Antibody, Mouse IgG2a (FAP5) at 1 μg/mL (100 μL/well) can bind Human FAP, Fc Tag (Cat. No. FAP-H5263) with a linear range of 0.5-4 ng/mL (Routinely tested).
Protocol
Bioactivity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >7000 pmol/min/µg (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Prolyl endopeptidase FAP
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
88 Details
- Highest R&D Stage：
Phase 3 Clinical
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