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Product Details
Synonyms
EPOR
Source
Human Erythropoietin R Protein, His Tag (EPR-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Pro 250 (Accession # P19235-1).
Predicted N-terminus: Ala 25
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 30-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human Erythropoietin R Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) is more than 85% and the molecular weight of this protein is around 25-38 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) at 5 μg/mL (100 μL/well) can bind Human Erythropoietin Protein, Fc Tag (Cat. No. EPO-H5253) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) immobilized on CM5 Chip can bind Human EPO Protein, premium grade (Cat. No. EPO-H4214) with an affinity constant of 0.142 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Bioactivity-CELL BASE
Human Erythropoietin R Protein, His Tag (Cat. No. EPR-H52H4) inhibits human erythropoietin/EPO-dependent proliferation of TF-1 cells. The EC50 is 211.1 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Erythropoietin receptor
- Category：
- Listed Drugs Count：
82 Details
- Clinical Drugs Count：
38 Details
- Highest R&D Stage：
Approved
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