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Product Details
Synonyms
TNFRSF25, DR3, APO3, DDR3, TNFRSF12, WSL, WSL1
Source
Human DR3 Protein, His Tag (TN5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.9 kDa. The protein migrates as 30-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human DR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) is more than 95% and the molecular weight of this protein is around 28-40 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with a linear range of 0.3-20 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with an affinity constant of 97.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 45.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) on SA Biosensor, can bind Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) with an affinity constant of 2.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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