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Mouse DR3 / TNFRSF25 Protein, His Tag, low endotoxin (HPLC & SPR verified)

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Product Number/Spec
Price
Menge
DR3-M52H3-100ug
$355.00
DR3-M52H3-1mg
$2335.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Synonyms

    TNFRSF25, DR3, APO3, DDR3, TNFRSF12, WSL, WSL1

  • Source

    Mouse DR3 Protein, His Tag (DR3-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Met 196 (Accession # B1AWN9-1).

    Request for sequence
  • Molecular Characterization

    DR3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 19.7 kDa. The protein migrates as 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • SDS-PAGE

    DR3 SDS-PAGE

    Mouse DR3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    DR3 SEC-HPLC

    The purity of Mouse DR3 Protein, His Tag (Cat. No. DR3-M52H3) was greater than 95% as determined by SEC-HPLC.

  • Bioactivity-SPR

     DR3 SPR

    Mouse DR3 Protein, His Tag (Cat. No. DR3-M52H3) immobilized on CM5 Chip can bind Mouse TL1A , His Tag (Cat. No. TLA-M5243) with an affinity constant of 17.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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Publish Comment

Background Introduction

Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.

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