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Product Details
Synonyms
EDSPD2
Source
Human Complement C1s Protein, His Tag (COS-H52H3) is expressed from human 293 cells (HEK293). It contains AA Glu 16 - Asp 688 (Accession # P09871).
Predicted N-terminus: Glu 16
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 76.8 kDa. The protein migrates as 27 kDa, 30 kDa, 40 kDa, 53-55 kDa and 90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Complement C1s Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Complement C1s Protein, His Tag (Cat. No. COS-H52H3) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.
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Bioactivity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >14,000 pmol/min/μg (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Complement C1s
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
Approved
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