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Product Details
Application
Synonyms
CLDN9
Source
Detergent Human Claudin-9, His,Twin-Strep Tag (CL9-H5586) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 217 (Accession # O95484-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.
The protein has a calculated MW of 27.9 kDa. The protein migrates as 23-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow the link for product information.
Structure
Each full-length transmembrane protein is encapsulated by detergent micelle.
Synthesis Process
Through mild detergent micelle extraction, dedicated solubilization, and stringent affinity purification, we maximally retain the native transmembrane conformation of full-length transmembrane proteins throughout downstream purification. This workflow yields purified, detergent-solubilized proteins free of unrelated membrane impurities and exogenous scaffold proteins, with exceptional purity, accurate quantitation, and well-preserved biological activity—ideal for immunization, binding assays in antibody discovery and screening (ELISA) and affinity testing (SPR and BLI), etc.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>80% as determined by SDS-PAGE.
Formulation
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human Claudin-9, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) at 5 μg/mL (100 μL/well) on a Nickel Coated plate can bind Anti-Claudin 9 Antibody with a linear range of 0.005-0.625 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Claudin 9 antibody captured on Protein G-Series S sensor chip can bind Human Claudin-9, His,Twin-Strep Tag (Cat. No. CL9-H5586) with an affinity constant of 38.8 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Claudin-9
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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