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Product Details
Application
Synonyms
Claudin-6, CLDN6
Source
Nanodisc-pro Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (CL6-H52M3) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Val 220 (Accession # P56747).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus.
The protein has a calculated MW of 54.0 kDa. The protein migrates as 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
*The isotype control of empty/mock nanodisc (Cat. No. APO-H5143) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc-pro membrane protein is wrapped by a piece of cell membrane and scaffold, the info of scaffold can be found in above red text "isotope type control...".
Synthesis Process
Nanodisc-pro membrane protein extraction is a gentle, non-disruptive process that directly isolates full-length transmembrane proteins together with their surrounding native phospholipids from cellular membranes, without breaking or disturbing the original membrane structure. This preserves the membrane protein's natural conformational landscape and native lipid environment, yielding stable nanodisc that retain cellular phospholipids, with fully preserved biological activity—making them ideal for cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA), etc.
Purity
>85% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE
Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.05-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) with an affinity constant of 1.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-FACS
Flow cytometric analysis of overexpressing Anti-Claudin-6(BBz) CAR-293 cells staining with Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) (2e5 of Anti-Claudin-6(BBz) CAR-293 cells were stained with 100 μL of 5μg/mL of Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag), compared with negative control protein analyzed with FACS (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Claudin 6
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
15 Details
- Highest R&D Stage：
Phase 2 Clinical
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